Ralph S. Pero, BS, MBA

7468 E. Nichols Place · Centennial, CO 80112 · (303)638-8639 · rpero@hotmail.com

OBJECTIVE

My goal is to use 16 years of scientific development and achievement in addition to the knowledge of a master’s degree education and business ownership to facilitate the business goals and success of a driven and dynamic business effort. Ultimately, I wish to find a fit that values the combination of my education in business administration and project implementation with business goals benefiting the customer, community, workforce and global society. 

Experience and education values include

Small Project Lead, Business Ownership, Finance and Accounting, Business Management, Transformational Leadership, Strategic Planning and Implementation, Corporate Finance, Operations Management, Business Law, Marketing, Statistics and Biostatistics, and Business Solutions

________________________________________________________________________________________

CURRENT POSITION

Senior Professional Research Assistant

University of Colorado School of Medicine

Department of Pharmacology

Mail Stop 8303

12801 E. 17th Ave, Room P18-6404F

Aurora, CO 80045

_________________________________________________________________________________________

EDUCATION

MBA, School of Business; 3.95GPA                                                                 University of Phoenix, Oct. 2010

BS, Molecular and Cellular Biology; Minor: Physics, Chemistry, Math          University of Arizona, Dec. 1994

Biostatistics, 12 lecture course                                                                                   University of Colorado, Oct. 2010

__________________________________________________________________________________________

WORK EXPERIENCE

University of Colorado – Sr. Professional Research Assistant, Aurora, Colorado 80045                 2008-Present

Report directly to the Lab Director to organize, design, and analyze experiments dealing with biological aspects of drug and alcohol dependency, characterizing genetic predisposition a novel small molecule to treat chronic pain disorders.

• Implemented a laboratory materials and ordering organization effort
• Participated in a major facility renovation requiring materials organization and collaborative success
• Developed and implemented biological procedures linked to grant applications for departmental financial stability, working under budgetary and time management constraints.

The Mayo Clinic, Senior Research Technologist II, Scottsdale, Arizona 85259                                  2002 – 2008

Reported directly to the program director to design, implement and interpret experimental results under resource constraints, to characterize a major cell constituent in the human immune system.  Personally responsible for materials and product management, detailed procedure implementation and accurate statistical result interpretation and reporting leading to peer reviewed journals and more than 2 million dollars in grand awards. 

• Designed, implemented and reported quality results leading to 8 secondary authorships of manuscripts published in high impact, peer reviewed journals in 6 years leading to program leadership, recognition, and respect in the industry.
• Generated data and wrote materials and methods sections for grant applications, leading to financial gains of more than $2 million in 6 years.
• Primary authorship of a manuscript published in a high impact, peer-reviewed journal which required development and collaboration of relationships with industry peers in a combined research initiative leading to program leadership in immunological pathway research
• Began and lead a collaborative effort in obtaining resources initiating brain tumor research and the relevance in immunology, defining the involvement of a particular white blood cell in brain cancer, creating a program entry point into cancer research.
• Lead a networking effort of immunological resource supply to collaborative research alliances globally and became an industry contact in eosinophil cell purification and interactions, often being sought for problem-solving consulting and procedure development assistance. 
• Developed into a comprehensive, program lead technologist, reporting results and progress through seminar and poster presentations at several international conferences, assisting with industry program branding and information sharing.

Allstate Insurance Agency, Investor, Owner and Treasurer                                                       2001-2006

Initiated and incorporated a business effort in insurance, investing $40,000 in an agency of 3-4 employees, successfully developing a customer base that increased the value of the agency to more than $200,000 in 5 years.

• Implemented direct marketing efforts creating sales and customer acquisition
• Created strong customer relationships and loyalty through customer contact, resulting in an agency with top customer retention and satisfaction ratings.
• Acquired an insurance agent certification through the state of Arizona (expired 2006)
• Sold the agency resulting in profits of $168,000 in 5 years.

The Barrow Neurological Institute, Senior Research Assistant                                                               1999-2002

Reported directly to the laboratory director to design, implement and report about experiments leading to efforts defining human brain tumors. 

• Secondary authorship of a manuscript published in a peer-reviewed journal assisting in grant funding applications
• Participated in community benefiting efforts through mentoring of several students in the laboratory

Myriad Genetics, Research Assistant I & II                                                                                             1995-1999

Join a team effort characterizing three cancer predisposition genes and leading to the development of several diagnostic tests detecting patient susceptibility to breast cancer.  The tests resulted in a sustainable income stream through negotiations with insurance companies and physicians. 

• Lead technologist performing genetic tests resulting in the characterization of an interaction with a major cancer susceptibility gene reported in a secondary authorship manuscript published in a peer-reviewed journal.

McLane Foods, Quality Assurance Laboratory Technician                                                                              1995

__________________________________________________________________________________________ 

TEACHING/MENTORING EXPERIENCE                                                                                    1999 - 2008

The Mayo Clinic and Barrow Neurological Institute, Taught and mentored 5 pre-medical students through hands-on training and lecture to design, implement and report the results of experiments leading to results needed for medical school applications.  All students were accepted and graduated from medical school, leading to profitable careers in the medical field.

__________________________________________________________________________________________

COMPUTER SKILLS

Basic knowledge with Excel including statistical manipulations and graphs, Word, PowerPoint, Outlook Money, Access, Adobe Photoshop, Graph pad, introduction to Sigma Plot, Internet browsers and searching, 40wpm.

PEER-REVIEWED PUBLICATIONS

Stefanie N. Linch, Ann M. Kelly, Erin T. Danielson, Ralph Pero, James J. Lee, Jeffrey A. Gold. Mouse Eosinophils Possess Potent Antibacterial Properties In Vivo.  In Press.

Eva Morschl, Jose G. Molina, Jonathan B. Volmer, Amir Mohsenin, Ralph S. Pero, Jeong-Soo Hong, Farrah Kheradmand, James J. Lee, and Michael R. Blackburn.  A3 Adenosine Receptor Signaling Influences Pulmonary Inflammation and Fibrosis.  Am J Respir Cell Mol Biol Vol 39. pp 697–705, 2008

Martina Ulrich, Alina Petre, Nikolay Youhnovski, Franziska Prömm, Markus Schirle, Michael Schumm, Ralph S. Pero, Alfred Doyle, James Checkel, Hirohito Kita, Nethaji Thiyagarajan, K. Ravi Acharya, Peter Schmid-Grendelmeier, Hans-Uwe Simon, Heinz Schwarz, Masato Tsutsui, Hiroaki Shimokawa, Gabriel Bellon, James J. Lee, Michael Przybylski, and Gerd Döring.  Post-translational tyrosine nitration of eosinophil granule toxins mediated by eosinophil peroxidase.  J. Biol. Chem. In press, August 11, 2008.

Elizabeth A. Jacobsen, Sergei I. Ochkur, Ralph S. Pero, Anna G. Taranova, Cheryl A. Protheroe, Dana C. Colbert, Nancy A. Lee, and James J. Lee.
Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells.  The Mayo Clinic, Scottsdale Arizona 85259. J. Exp. Med., Mar 2008; 205: 699 - 710.

Nancy Lee, Cheryl Protheroe, Sergei Ochkur, Elizabeth Jacobsen, Ralph Pero, Anna Taranova, Dana Colbert, Katie O’Neill, Alfred Doyle, Margaret McGarry, Kavithe Kesarkar, Marck V. Dahl, Leroy Griffing, Jamie J. Lee.  Eosinophil expression of IL-4 cures cancer and causes atopy.  The Mayo Clinic, Scottsdale Arizona 85259, Science, in review

Sergei I. Ochkur, Elizabeth A. Jacobsen, Cheryl A. Protheroe, Travis L. Biechele, Ralph  

            S. Pero, Michael P. McGarry, Huiying Wang, Katie R. O’Neill, Dana C. Colbert,

            Thomas V. Colby, Huahao Shen, Michael R. Blackburn, Charles C. Irvine, James

            J. Lee, and Nancy A. Lee.  Coexpression of IL-5 and Eotaxin-2 in Mice

            Creates an Eosinophil-Dependent Model of Respiratory Inflammation with

            Characteristics of Severe Asthma.  The Mayo Clinic, Scottsdale, Arizona

85259, Journal of Immunology vol 178, No.12, June 15, 2007, 7879-7889.

Ralph S. Pero, Michael T. Borchers, Karsten Spicher, Sergei I. Ochkur, Lyudmila, Sikora, Savita P. Rao, Hiam Abdala-Valencia, Katie R. O’Neill, Huahao Shen, Melvin I.Simon, Michael P. McGarry, Nancy A. Lee, Joan M. Cook-Mills, P. Sriramarao,Lutz Birnbaumer, and James J. Lee.  Extravasation of Leukocytes from Circulation is Controled by Ga_i2 Signaling Events in the Endothelium.  The Mayo Clinic, Scottsdale, Arizona 85259, PNAS vol.104, no.11, March 13, 2007, 4371-4376.

Stephania A. Cormier, Anna G. Taranova, Carrie Bedient, Thanh Nguyen, Cheryl

Protheroe, Ralph Pero, Dawn Dimina, Sergei I. Ochkur, Katie O’Neill, Dana

Colbert, Theresa R. Lombari, Stephanie Constant, Michael P. McGarry, James J. Lee, and Nancy A. Lee.  Pivotal Advance: Eosinophil Infiltration of Solid Tumors is an Early and Persistent Inflammatory Host Response.  The mayo Clinic, Scottsdale, Arizona 85259, Journal of Leukocyte Biology vol 79, June 2006: 1131-1139

James J. Lee, Dawn Dimina, MiMi P. Macias, Sergei I. Ochkur, Michael P. McGarry,

Katie R. O'Neill, Cheryl Protheroe, Ralph Pero, Thanh Nguyen, Stephania A.

Cormier, Elizabeth Lenkiewicz, Dana Colbert, Lisa Rinaldi, Steven J. Ackerman,

Charles G. Irvin, and Nancy A. Lee.  Defining a Link with Asthma in Mice

Congenitally Deficient in Eosinophils.  The Mayo Clinic, Scottsdale, AZ 85259.

Science vol 305, 17 September 2004: 1773-1776.

Ivanov, Andrei I., Pero, Ralph S., Scheck, Adrienne C. and Romanovsky, Andrej A.

2002.  Prostaglandin E₂-synthesizing Enzymes in Lipopolysaccharide Fever:  Differential Transcriptional Regulation at Different Febrile Phases.  Trauma Research andNeurology Research, Barrow Neurological Institute, St. Joseph’s  Hospital andMedical Center, Phoenix, Arizona 85013.  Am J Physiol Regul Integr Comp Physiol 283: R1104-R1117, 2002.

Adey NB, Huang L, Ormonde PA, Baumgard ML, Pero R, Byreddy DV, Tavtigian SV,

Bartel PL.  2000.  Threonine phosphorylation of the MMAC1/PTEN PDZ binding domain both inhibits and stimulates PDZ binding. Cancer Res 2000 Jan 1;60(1):35-7.

Alexander, K.C. Wong, Patricia A. Ormonde, Ralph Pero, Yuan Chen, Lubing Lian, Grant Salada, Simin Berry, Quentin Lawrence, Priya Dayananth, Phoung Ha, Sean V. Tavtigian, David H-F. Teng and Paul L. Bartel.  1998. Characterization of a carboxy-terminal BRCA1 interacting protein. Oncogene17:  2279-2285

Alexander K.C. Wong, Ralph Pero, Patricia A. Ormonde, Sean Tavtigian, and Paul L.     Bartel. 1996.  RAD51 Interacts with the Evolutionary Conserved BRC Motifs in the Human Breast Susceptibility Gene BRCA2. J.  Biol. Chem. 272:  31941-

31944

David H-F. Teng, William L. Perry III, James K. Hogan, Michelle Baumgard, Russel        Bell, Simin Berry, Thaylon Davis, David Frank, Cheryl Frye, Thomas Hattier,    Rong Hu, Srikanth Jammulapati, Teresa Janecki, Amber Leavitt, Jeffrey T.       Mitchell, Ralph Pero, David Sexton, Marianne Schroeder, Pi-Hsia Su, Brad             Swedlund, John M. Kyriakis, Joseph Avruch, Paul Bartel, Alexander K.C. Wong,             Arnold Oliphant, Alun Thomas, Mark H. Skolnick, and Sean V. Tavtigian.  1996.              Human Mitogen-activated Protein Kinase Kinase 4 as a Candidate Tumor            Suppressor. Cancer Research.  57:  4177-4182.     

REFERENCES AVAILABLE UPON REQUEST